cloned sequence meaning in Chinese
克隆片段
克隆序列
Examples
- And many important cis - acting regulatory elements were found in the cloned sequence region by plantcare software analysis . ref promoter was predicted to be most probably induced by light , hot , gibberellin , ethenen or wound
经plantcare软件分析发现序列中含有多种调控元件，经预测ref启动子可能被光、热、 ga 、乙烯或伤所诱导。 - The activity of the invertase of three high - sugar saccharomyces cerevisiae strains were investigated and their amino acid sequence were determined by the use of modern biotech including enzymatic activity determination , specific pcr , the cloning sequencing and bio - information analysis etc
摘要采用现代生物技术中的酶活测定、特异pcr 、克隆测序和生物信息分析等对比研究3株高糖酵母蔗糖转换酶活性及其氨基酸序列。 - Two test sets were constructed by mapping the newly published 28 , 469 full - length kome rice cdna to the rgp bac clone sequences of oryza sativa ssp . japonica : a single - gene set of 550 sequences and a multi - gene set of 62 sequences with 271 genes
我们把日本kome数据库中的28469个全长cdna同国际水稻基因组计划的粳稻bac序列对比，经过严格选择构建了两个测试数据集合： 550条单基因序列组成ossng550集合，包含271个基因的62条多基因序列组成osmtg62集合。 - After electrophorised on 1 % agarose gel , the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a . a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion , pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12 - 16 hours of culture , several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction , mstnd - pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
F _ 1长38bp ， r _ 1长36bp ，其它片段均40bp长， f _ 1和r _ 1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18 - t载体连接、转化dh _ 5 。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18 - t载体上的特异引物rv - m和m13 - 47进行pcr鉴定，获得300bp的片段。 - In order to use the responding ability to the inducers of pr - la promoter , two fragments , ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank . sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence . its transcription start site , tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter
根据pr - 1a基因的报道序列，设计两对引物，以烟草基因组为模板，通过pcr扩增得到900bp ( ipl )和603bp ( ips )两个目标片段，序列测定表明克隆的启动子与报道序列具有99的同源性，转录起始位点、 tatabox及保守序列tgac与报道序列均完全相同。